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secondary goat anti rabbit ir 680  (LI-COR)


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    Structured Review

    LI-COR secondary goat anti rabbit ir 680
    Secondary Goat Anti Rabbit Ir 680, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rabbit+ir+680/pmc10399470-148-5-23
    Average 86 stars, based on 1 article reviews
    secondary goat anti rabbit ir 680 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Overcoming Amino-Nogo-induced Inhibition of Cell Spreading and Neurite Outgrowth by 12- O -Tetradecanoylphorbol-13-acetate-type Tumor Promoters
    Article Snippet: Proteins were then transferred to nitrocellulose membrane (Bio-Rad) using the Mini Trans-Blot® electrophoretic transfer cell system (Bio-Rad) at 100 V for 1 h. Membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences) at room temperature for 1 h and then incubated with primary antibodies diluted in Odyssey blocking buffer with 0.05% Tween 20 overnight at 4 °C. .. The membranes were washed with PBS, 0.1% Tween 20 four times at room temperature and stained with secondary goat anti-rabbit IR 680 and goat anti-mouse IR 800 (Li-COR Biosciences) in Odyssey blocking buffer with 0.05% Tween 20 at room temperature for 1 h. After four more rinses, membranes were scanned with an Odyssey infrared imaging system. ..

    Article Title: Overcoming Amino-Nogo-induced Inhibition of Cell Spreading and Neurite Outgrowth by 12-O-Tetradecanoylphorbol-13-acetate-type Tumor Promoters
    Article Snippet: Proteins were then transferred to nitrocellulose membrane (Bio-Rad) using the Mini Trans-Blot electrophoretic transfer cell system (Bio-Rad) at 100 V for 1 h. Membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences) at room temperature for 1 h and then incubated with primary antibodies diluted in Odyssey blocking buffer with 0.05% Tween 20 overnight at 4 °C. .. The membranes were washed with PBS, 0.1% Tween 20 four times at room temperature and stained with secondary goat anti-rabbit IR 680 and goat anti-mouse IR 800 (Li-COR Biosciences) in Odyssey blocking buffer with 0.05% Tween 20 at room temperature for 1 h. After four more rinses, membranes were scanned with an Odyssey infrared imaging system. ..

    Blocking Assay:

    Article Title: Overcoming Amino-Nogo-induced Inhibition of Cell Spreading and Neurite Outgrowth by 12- O -Tetradecanoylphorbol-13-acetate-type Tumor Promoters
    Article Snippet: Proteins were then transferred to nitrocellulose membrane (Bio-Rad) using the Mini Trans-Blot® electrophoretic transfer cell system (Bio-Rad) at 100 V for 1 h. Membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences) at room temperature for 1 h and then incubated with primary antibodies diluted in Odyssey blocking buffer with 0.05% Tween 20 overnight at 4 °C. .. The membranes were washed with PBS, 0.1% Tween 20 four times at room temperature and stained with secondary goat anti-rabbit IR 680 and goat anti-mouse IR 800 (Li-COR Biosciences) in Odyssey blocking buffer with 0.05% Tween 20 at room temperature for 1 h. After four more rinses, membranes were scanned with an Odyssey infrared imaging system. ..

    Article Title: Overcoming Amino-Nogo-induced Inhibition of Cell Spreading and Neurite Outgrowth by 12-O-Tetradecanoylphorbol-13-acetate-type Tumor Promoters
    Article Snippet: Proteins were then transferred to nitrocellulose membrane (Bio-Rad) using the Mini Trans-Blot electrophoretic transfer cell system (Bio-Rad) at 100 V for 1 h. Membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences) at room temperature for 1 h and then incubated with primary antibodies diluted in Odyssey blocking buffer with 0.05% Tween 20 overnight at 4 °C. .. The membranes were washed with PBS, 0.1% Tween 20 four times at room temperature and stained with secondary goat anti-rabbit IR 680 and goat anti-mouse IR 800 (Li-COR Biosciences) in Odyssey blocking buffer with 0.05% Tween 20 at room temperature for 1 h. After four more rinses, membranes were scanned with an Odyssey infrared imaging system. ..

    Article Title: Mysm1 mutations in meander tail mice cause anterior-selective cerebellum malformation
    Article Snippet: 20 μg of protein from mutant and control littermate samples were fractionated on 8% acrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, 1620115). .. After blocking with 5% non-fat dry milk in PBST, membranes were exposed to rabbit anti-MYSM1 antibody (Invitrogen PA5-102696, raised against residues 645-695 relative to Q5VVJ2, including the JAMM motif) and mouse anti-GAPDH (GeneTex GTX627408) in blocking solution for 1 hour at room temperature, washed 4x in blocking solution, then exposed to goat anti-rabbit IR-680 (Li-Cor 92568071) and goat anti-mouse IR-800 (Li-Cor 92632210) dye-conjugated secondary antibodies for 1 hour at room temperature, and washed once in blocking solution, twice in PBST and once in PBS. .. Fluorescence was quantified on a LI-COR Odyssey instrument with ImageStudio software (LiCOR).

    Imaging:

    Article Title: Overcoming Amino-Nogo-induced Inhibition of Cell Spreading and Neurite Outgrowth by 12- O -Tetradecanoylphorbol-13-acetate-type Tumor Promoters
    Article Snippet: Proteins were then transferred to nitrocellulose membrane (Bio-Rad) using the Mini Trans-Blot® electrophoretic transfer cell system (Bio-Rad) at 100 V for 1 h. Membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences) at room temperature for 1 h and then incubated with primary antibodies diluted in Odyssey blocking buffer with 0.05% Tween 20 overnight at 4 °C. .. The membranes were washed with PBS, 0.1% Tween 20 four times at room temperature and stained with secondary goat anti-rabbit IR 680 and goat anti-mouse IR 800 (Li-COR Biosciences) in Odyssey blocking buffer with 0.05% Tween 20 at room temperature for 1 h. After four more rinses, membranes were scanned with an Odyssey infrared imaging system. ..

    Article Title: Overcoming Amino-Nogo-induced Inhibition of Cell Spreading and Neurite Outgrowth by 12-O-Tetradecanoylphorbol-13-acetate-type Tumor Promoters
    Article Snippet: Proteins were then transferred to nitrocellulose membrane (Bio-Rad) using the Mini Trans-Blot electrophoretic transfer cell system (Bio-Rad) at 100 V for 1 h. Membranes were blocked with Odyssey blocking buffer (Li-COR Biosciences) at room temperature for 1 h and then incubated with primary antibodies diluted in Odyssey blocking buffer with 0.05% Tween 20 overnight at 4 °C. .. The membranes were washed with PBS, 0.1% Tween 20 four times at room temperature and stained with secondary goat anti-rabbit IR 680 and goat anti-mouse IR 800 (Li-COR Biosciences) in Odyssey blocking buffer with 0.05% Tween 20 at room temperature for 1 h. After four more rinses, membranes were scanned with an Odyssey infrared imaging system. ..



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